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Cell. 2015 Nov 5;163(4):799-810. doi: 10.1016/j.cell.2015.10.039.

Design and Analysis of Single-Cell Sequencing Experiments.

Author information

1
Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences), 3584 CT Utrecht, the Netherlands; University Medical Center Utrecht, Cancer Genomics Netherlands, 3584 CX Utrecht, the Netherlands; Max Planck Institute of Immunobiology and Epigenetics, D-79108 Freiburg, Germany.
2
Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences), 3584 CT Utrecht, the Netherlands; University Medical Center Utrecht, Cancer Genomics Netherlands, 3584 CX Utrecht, the Netherlands. Electronic address: a.vanoudenaarden@hubrecht.eu.

Abstract

Recent advances in single-cell sequencing hold great potential for exploring biological systems with unprecedented resolution. Sequencing the genome of individual cells can reveal somatic mutations and allows the investigation of clonal dynamics. Single-cell transcriptome sequencing can elucidate the cell type composition of a sample. However, single-cell sequencing comes with major technical challenges and yields complex data output. In this Primer, we provide an overview of available methods and discuss experimental design and single-cell data analysis. We hope that these guidelines will enable a growing number of researchers to leverage the power of single-cell sequencing.

PMID:
26544934
DOI:
10.1016/j.cell.2015.10.039
[Indexed for MEDLINE]
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