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New Phytol. 2016 Mar;209(4):1625-40. doi: 10.1111/nph.13735. Epub 2015 Nov 6.

Differentially expressed small RNAs in Arabidopsis galls formed by Meloidogyne javanica: a functional role for miR390 and its TAS3-derived tasiRNAs.

Author information

1
Universidad de Castilla-La Mancha, Facultad de Ciencias Ambientales y Bioquímica, Avda. Carlos III, s/n 45071, Toledo, Spain.
2
Molecular Cytogenetics Group, Human Cancer Genetics Programme, Centro Nacional Investigaciones Oncológicas (CNIO), C/Melchor Fernández Almagro, 3, 28029 , Madrid, Spain.
3
INRA, Université Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia Agrobiotech, 06900, Sophia Antipolis, France.
4
Centre for Organismal Studies University of Heidelberg, Im Neuenheimer Feld, 230-69120, Heidelberg, Germany.
5
Centro de Investigaciones Biológicas, CSIC, Av. Ramiro de Maeztu 9, 28040, Madrid, Spain.

Abstract

Root-knot nematodes (RKNs) induce inside the vascular cylinder the giant cells (GCs) embedded in the galls. The distinctive gene repression in early-developing GCs could be facilitated by small RNAs (sRNA) such as miRNAs, and/or epigenetic mechanisms mediated by 24nt-sRNAs, rasiRNAs and 21-22nt-sRNAs. Therefore, the sRNA-population together with the role of the miR390/TAS3/ARFs module were studied during early gall/GC formation. Three sRNA libraries from 3-d-post-inoculation (dpi) galls induced by Meloidogyne javanica in Arabidopsis and three from uninfected root segments were sequenced following Illumina-Solexa technology. pMIR390a::GUS and pTAS3::GUS lines were assayed for nematode-dependent promoter activation. A sensor line indicative of TAS3-derived tasiRNAs binding to the ARF3 sequence (pARF3:ARF3-GUS) together with a tasiRNA-resistant ARF3 line (pARF3:ARF3m-GUS) were used for functional analysis. The sRNA population showed significant differences between galls and controls, with high validation rate and correspondence with their target expression: 21-nt sRNAs corresponding mainly to miRNAs were downregulated, whilst 24-nt-sRNAs from the rasiRNA family were mostly upregulated in galls. The promoters of MIR390a and TAS3, active in galls, and the pARF3:ARF3-GUS line, indicated a role of TAS3-derived-tasiRNAs in galls. The regulatory module miR390/TAS3 is necessary for proper gall formation possibly through auxin-responsive factors, and the abundance of 24-nt sRNAs (mostly rasiRNAs) constitutes a gall hallmark.

KEYWORDS:

galls; giant cells; meloidogyne; miRNAs; rasiRNAs; silencing; small RNAs; tasiRNAs

PMID:
26542733
DOI:
10.1111/nph.13735
[Indexed for MEDLINE]
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