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Sci Rep. 2015 Nov 6;5:16248. doi: 10.1038/srep16248.

Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody.

Author information

1
INSERM, UMR S 1138, Centre de Recherche des Cordeliers, Paris, France.
2
Université Pierre et Marie Curie-Paris6, UMR S 1138, Paris, France.
3
Université Paris Descartes, UMR S 1138, Paris, France.
4
Flavivirus-Host Molecular Interactions laboratory, Virology Department, Institut Pasteur, Paris, France.
5
Vaccine and Infectious Disease Research Centre, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
6
Deutsches Rheuma-Forschungszentrum, Institut der Leibniz-Gemeinschaft, 10117 Berlin, Germany.
7
Infection and Epidemiology Department, Institut Pasteur, 75724 Paris &UMR PIMIT (I2T), University of Reunion Island, INSERM U1187, CNRS 9192, IRD 249, GIP-CYROI, la Reunion, France.

Abstract

Geographical expansion and re-emerging new genotypes of the Japanese encephalitis virus (JEV) require the development of novel therapeutic approaches. Here, we studied a non-conventional approach for antibody therapy and show that, upon exposure to heme, a fraction of natural human immunoglobulins acquires high-affinity reactivity with the antigenic domain-III of JEV E glycoprotein. These JEV-reactive antibodies exhibited neutralizing activity against recently dominant JEV genotypes. This study opens new therapeutic options for Japanese encephalitis.

PMID:
26542535
PMCID:
PMC4635365
DOI:
10.1038/srep16248
[Indexed for MEDLINE]
Free PMC Article

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