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Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2015 Oct;40(10):1060-7.DOI:10.11817/j.issn.1672-7347.2015.10.002.

[MicroRNA-33a regulates the invasion of cervical cancer cells via targeting Twist1].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

1
Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha 410013;. Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
2
Department of Pediatrics, Second Hospital of 
Xi'an Jiaotong University, Xi'an 710004, China.
3
School of Basic Medical Science, Central South University, Changsha 410013, China.
4
Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha 410013, China.

Abstract

in English, Chinese

OBJECTIVE:

To examine the expression of Twist1 in cervical cancer and to explore its biological function in the progression of cervical cancer.


METHODS:

The expressions of Twist1 in 32 cervical cancers and matched normal tissues were examined by immunohistochemistry (IHC). Cell invasive ability and the expression of invasion-related genes were determined in RNAi-based Twist1-silencing HeLa cells. The relationship between Twist1 and microRNA-33a (miR-33a) in cervical cancer was studied by Pearson correlation analysis, and the roles of miR-33a in regulation of Twist1 and cell invasiveness were studied.


RESULTS:

The positive expression rate of Twist1 was 75.0% (24/32) and 21.9% (7/32) in the cervical cancer and the matched normal tissues, respectively, with significant difference between them (P<0.05). Twist1 shRNA significantly decreased the invasiveness of HeLa cells (P<0.05). Compared with the matched normal tissues, the expression of miR-33a was increased in the cervical cancer tissues, which was negatively correlated with Twist1 (r=-0.661, P<0.05). Overexpression of miR-33a could significantly suppress Twist1 expression as well as cell invasiveness (P<0.05).


CONCLUSION:

Twist1 is critical for the invasiveness of cervical cancer cells; miR-33a, as a tumor suppressor gene, functions as an upstream regulator of Twist1 and is involved in the invasiveness of cervical cancer cell.

[Indexed for MEDLINE]
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