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Nat Commun. 2015 Nov 6;6:8717. doi: 10.1038/ncomms9717.

Cytokinin response factors regulate PIN-FORMED auxin transporters.

Author information

1
Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Gent, Belgium.
2
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, B-9052 Gent, Belgium.
3
Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria.
4
Department of Developmental Genetics, Centre for Molecular Biology of Plants, University Tübingen, Auf der Morgenstelle 3, 72076 Tübingen, Germany.
5
Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, the Netherlands.
6
Institute of Experimental Botany, ASCR, Rozvojová 263, 16502 Prague, Czech Republic.
7
Department of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland 20742, USA.

Abstract

Auxin and cytokinin are key endogenous regulators of plant development. Although cytokinin-mediated modulation of auxin distribution is a developmentally crucial hormonal interaction, its molecular basis is largely unknown. Here we show a direct regulatory link between cytokinin signalling and the auxin transport machinery uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory element effectively uncouples PIN transcription from the CRF-mediated cytokinin regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent a missing cross-talk component that fine-tunes auxin transport capacity downstream of cytokinin signalling to control plant development.

PMID:
26541513
DOI:
10.1038/ncomms9717
[Indexed for MEDLINE]

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