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J Exp Clin Cancer Res. 2015 Nov 4;34:135. doi: 10.1186/s13046-015-0250-6.

Linc00152 promotes proliferation in gastric cancer through the EGFR-dependent pathway.

Author information

1
Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, P.R. China. staff882@yxph.com.
2
Department of Gastrointestinal Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu, P.R. China. staff882@yxph.com.
3
Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, Jiangsu, P.R. China.
4
Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
5
Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, P.R. China. xuzekuan@njmu.edu.cn.

Abstract

BACKGROUND:

Linc00152 has been identified highly associated with the tumorigenesis and development of gastric cancer, however, the detailed mechanism of Linc00152 involved still remains unclear.

METHODS:

RT-PCR and western blot were used to detect the expression of Linc00152 and EGFR. The CCK8 and EDU assay was employed to measure cell proliferation while xenotransplantation technology was applied in BALB/C nude mice. The interaction between lncRNA and target protein was investigated by RNA pull-down and RNA immunoprecipitation assay.

RESULTS:

In this study, we first confirmed the upregulation of cytoplasmic expressed Linc00152 in 72 pair tissues of gastric patients. A suppression of cell proliferation and tumor growth was obtained in MGC803 and HGC-27 cells treated with Linc00152 shRNA. RNA pull-down and RIP assay revealed that Linc00152 could directly bind with EGFR which caused an activation of PI3K/AKT signaling.

CONCLUSION:

We first found that Linc00152 could promote tumor growth through EGFR-mediated PI3K/AKT pathway which may serve as potential targets for therapy in the future.

PMID:
26538117
PMCID:
PMC4632266
DOI:
10.1186/s13046-015-0250-6
[Indexed for MEDLINE]
Free PMC Article

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