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J Clin Microbiol. 2016 Jan;54(1):204-7. doi: 10.1128/JCM.02047-15. Epub 2015 Nov 4.

Performance of BD Max StaphSR for Screening of Methicillin-Resistant Staphylococcus aureus Isolates among a Contemporary and Diverse Collection from 146 Institutions Located in Nine U.S. Census Regions: Prevalence of mecA Dropout Mutants.

Author information

1
JMI Laboratories, North Liberty, Iowa, USA rodrigo-mendes@jmilabs.com.
2
JMI Laboratories, North Liberty, Iowa, USA.

Abstract

This study determined the performance of BD Max StaphSR and the rate of methicillin-resistant Staphylococcus aureus (MRSA) with an unrecognized staphylococcal cassette chromosome mec (SCCmec) right-extremity junction (MREJ) region among 907 methicillin-resistant S. aureus (MRSA) and 900 methicillin-susceptible S. aureus (MSSA) isolates. The rate of mecA/mecC dropout mutants was also evaluated. Only three MRSA isolates (99.7% sensitivity; 904/907) were classified as MSSA by the BD Max StaphSR assay, due to negative results for MREJ. Eight MSSA isolates (99.1% sensitivity; 892/900) were assigned as MRSA. However, six of these MSSA isolates had the mecA gene confirmed by PCR and sequencing (99.8% sensitivity; 898/900). Overall, 7.1% (64/900) of MSSA isolates showed results compatible with a mecA dropout genotype.

PMID:
26537444
PMCID:
PMC4702731
DOI:
10.1128/JCM.02047-15
[Indexed for MEDLINE]
Free PMC Article

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