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Biochim Biophys Acta. 1989 May 15;1003(1):15-9.

Accumulation of 14 alpha-methylergosta-8,24(28)-dien-3 beta,6 alpha-diol in 14 alpha-demethylation mutants of Candida albicans: genetic evidence for the involvement of 5-desaturase.

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1
Department of Microbiology, School of Dentistry, Kyushu University, Fukuoka, Japan.

Abstract

14 alpha-Demethylation mutants of the fungus Candida albicans have been shown to accumulate 14 alpha-methylergosta-8,24(28)-dien-3 beta,6 alpha-diol. A derivative from one of these mutants (KD4900) that does not form this 6 alpha-hydroxylated sterol but is still defective in 14 alpha-demethylation (KD4950) was obtained. Mutational restitution of 14 alpha-demethylation capacity to this derivative resulted in the formation of the 5,6-saturated sterol ergosta-7,22-dien-3 beta-ol as the major product, clearly indicating that 5-desaturase deficiency exists in this demethylation-proficient revertant (KD4952). This implies that its parent, KD4950, which has lost the ability to form the hydroxylated sterol, also is deficient in 5-desaturation. We infer from the results that 5-desaturase is responsible for the formation of the hydroxylated sterol. However, it is unclear whether the hydroxylation represents a genuine step of the normal 5-desaturation reaction.

PMID:
2653443
DOI:
10.1016/0005-2760(89)90092-1
[Indexed for MEDLINE]

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