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Methods Mol Biol. 2016;1372:61-77. doi: 10.1007/978-1-4939-3148-4_5.

Analyses of Tumor Burden In Vivo and Metastasis Ex Vivo Using Luciferase-Expressing Cancer Cells in an Orthotopic Mouse Model of Neuroblastoma.

Author information

1
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
2
Tumour Biology and Targeting Program, Children's Cancer Institute, Lowy Cancer Research Centre, University of New South Wales, Randwick, NSW, Australia.
3
ARC Centre of Excellence in Convergent Bio-Nano Science, Australian Centre for Nanomedicine, University of New South Wales, Randwick, NSW, Australia.
4
Tumour Biology and Targeting Program, Children's Cancer Institute, Lowy Cancer Research Centre, University of New South Wales, Randwick, NSW, Australia. mkavallaris@ccia.unsw.edu.au.
5
ARC Centre of Excellence in Convergent Bio-Nano Science, Australian Centre for Nanomedicine, University of New South Wales, Randwick, NSW, Australia. mkavallaris@ccia.unsw.edu.au.

Abstract

Cancer xenograft mouse models are useful for examining and understanding tumor growth and cancer progression in vivo. With the development of bioluminescent imaging, these parameters can now be monitored noninvasively with relative ease. Herein we describe imaging of luciferase-expressing cancer cells to quantitatively measure tumor burden in vivo and metastases ex vivo. Specifically, we detail the methodology to examine the effect of shRNA-mediated knockdown of a target gene on the growth and spread of neuroblastoma tumors in immune-deficient mice.

KEYWORDS:

Luciferase; Metastasis; Neuroblastoma; Orthotopic; Short hairpin RNA

PMID:
26530915
DOI:
10.1007/978-1-4939-3148-4_5
[Indexed for MEDLINE]

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