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Methods Mol Biol. 2016;1371:225-38. doi: 10.1007/978-1-4939-3139-2_15.

Standardized Multi-Color Flow Cytometry and Computational Biomarker Discovery.

Author information

1
Institute of Medical Immunology, Charité University Medicine, Berlin, 10117, Germany.
2
Institute of Medical Immunology, Charité University Medicine, Berlin, 10117, Germany. birgit.sawitzki@charite.de.

Abstract

Multi-color flow cytometry has become a valuable and highly informative tool for diagnosis and therapeutic monitoring of patients with immune deficiencies or inflammatory disorders. However, the method complexity and error-prone conventional manual data analysis often result in a high variability between different analysts and research laboratories. Here, we provide strategies and guidelines aiming at a more standardized multi-color flow cytometric staining and unsupervised data analysis for whole blood patient samples.

KEYWORDS:

Data analysis; Flow cytometry; Immune monitoring; Standardization

PMID:
26530805
DOI:
10.1007/978-1-4939-3139-2_15
[Indexed for MEDLINE]

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