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EMBO J. 2015 Dec 14;34(24):2993-3008. doi: 10.15252/embj.201592409. Epub 2015 Nov 3.

Subunit composition of VRAC channels determines substrate specificity and cellular resistance to Pt-based anti-cancer drugs.

Author information

1
Leibniz-Institut für Molekulare Pharmakologie (FMP) Max-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin, Germany.
2
Division of Molecular Oncology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
3
Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
4
Division of Molecular Pathology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
5
Division of Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
6
Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
7
Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland Division of Molecular Pathology, The Netherlands Cancer Institute, Amsterdam, The Netherlands sven.rottenberg@vetsuisse.unibe.ch Jentsch@fmp-berlin.de.
8
Leibniz-Institut für Molekulare Pharmakologie (FMP) Max-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin, Germany NeuroCure Cluster of Excellence, Charité Universitätsmedizin, Berlin, Germany sven.rottenberg@vetsuisse.unibe.ch Jentsch@fmp-berlin.de.

Abstract

Although platinum-based drugs are widely used chemotherapeutics for cancer treatment, the determinants of tumor cell responsiveness remain poorly understood. We show that the loss of subunits LRRC8A and LRRC8D of the heteromeric LRRC8 volume-regulated anion channels (VRACs) increased resistance to clinically relevant cisplatin/carboplatin concentrations. Under isotonic conditions, about 50% of cisplatin uptake depended on LRRC8A and LRRC8D, but neither on LRRC8C nor on LRRC8E. Cell swelling strongly enhanced LRRC8-dependent cisplatin uptake, bolstering the notion that cisplatin enters cells through VRAC. LRRC8A disruption also suppressed drug-induced apoptosis independently from drug uptake, possibly by impairing VRAC-dependent apoptotic cell volume decrease. Hence, by mediating cisplatin uptake and facilitating apoptosis, VRAC plays a dual role in the cellular drug response. Incorporation of the LRRC8D subunit into VRAC substantially increased its permeability for cisplatin and the cellular osmolyte taurine, indicating that LRRC8 proteins form the channel pore. Our work suggests that LRRC8D-containing VRACs are crucial for cell volume regulation by an important organic osmolyte and may influence cisplatin/carboplatin responsiveness of tumors.

KEYWORDS:

VSOAC; VSOR; chloride channel; haploid cell screen; swelling‐activated

Comment in

PMID:
26530471
PMCID:
PMC4687416
DOI:
10.15252/embj.201592409
[Indexed for MEDLINE]
Free PMC Article

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