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J Chromatogr A. 2015 Dec 4;1423:79-85. doi: 10.1016/j.chroma.2015.10.064. Epub 2015 Oct 25.

Qualitative and quantitative analysis of branches in dextran using high-performance anion exchange chromatography coupled to quadrupole time-of-flight mass spectrometry.

Author information

1
Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215021, China.
2
Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8(th) Street, Troy, NY 12180, USA.
3
Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215021, China. Electronic address: z_zhang@suda.edu.cn.

Abstract

Dextran, a family of natural polysaccharides, consists of an α (1→6) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important in understanding its various biological roles. In this study, a hyphenated method using high-performance anion exchange chromatography (HPAEC) in parallel with pulsed amperometric detection (PAD) and mass spectrometry (MS) was applied to qualitative and quantitative analysis of dextran branches. A rotary cation-exchange cartridge array desalter was used for removal of salt from the HPAEC eluent making it MS compatible. MS and MS/MS were used to provide structural information on the enzymatically prepared dextran oligosaccharides. PAD provides quantitative data on the ratio of enzyme-resistant, branched dextran oligosaccharides. Both the types and degree of branching found in a variety of dextrans could be simultaneously determined online using this method.

KEYWORDS:

Branches; Dextran; HPAEC-MS; Qualitative analysis; Quantitative analysis

PMID:
26530145
DOI:
10.1016/j.chroma.2015.10.064
[Indexed for MEDLINE]

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