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J Cell Biochem. 2016 Jun;117(6):1352-8. doi: 10.1002/jcb.25425. Epub 2015 Nov 16.

Recombinant Mitochondrial Manganese Containing Superoxide Dismutase Protects Against Ochratoxin A-Induced Nephrotoxicity.

Author information

1
Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", 80137 Naples, Italy.
2
Department of Nephrology, Second University of Naples, Naples, Italy.
3
Molecular Biology and Viral Oncology Unit, Department of Experimental Oncology, Istituto Nazionale Tumori, "Fondazione G. Pascale", IRCCS, Naples, Italy.
4
Leadhexa Biotechnologies Inc., San Francisco, California.
5
Sbarro Institute for Cancer Research and Molecular Medicine, Center of Biotechnology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania.
6
Department of Medicine, Surgery and Neuroscience, University of Siena, Siena, Italy.

Abstract

Ochratoxin A (OTA) is a natural mycotoxin, involved in the development of important human and animal diseases. In this work we have studied the role of oxidative stress in the development of OTA nephrotoxicity and the effect of a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) to prevent kidney damage induced by OTA. Blood pressure, glomerular filtration rate and renal histology were analyzed in control rats and in OTA treated rats. In addition, lipid peroxidation, catalase and superoxide dismutase productions were measured. Our data showed that animals treated with OTA presented hypertension and reduction of glomerular filtration rate (GFR). These effects are most probably related to an increase in the reactive oxygen species (ROS) productions. In fact, we have shown that treatment with rMnSOD restored the levels of blood pressure and GFR simultaneously. Moreover, we have noted that OTA induced alteration on glomerular and tubular degeneration and interstitial infiltrates and that use of rMnSOD combined with OTA prevent this renal histological damage confirming the potential therapeutic role in the treatment of rMnSOD OTA nephrotoxicity.

KEYWORDS:

NEPHROTOXICITY; OCHRATOXIN A; OXIDATIVE STRESS; rMnSOD

PMID:
26529273
DOI:
10.1002/jcb.25425
[Indexed for MEDLINE]

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