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Int J Biol Macromol. 2016 Jan;82:973-8. doi: 10.1016/j.ijbiomac.2015.10.088. Epub 2015 Nov 1.

Activation effect of Ganoderma lucidum polysaccharides liposomes on murine peritoneal macrophages.

Author information

1
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
2
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China. Electronic address: dywang@njau.edu.cn.

Abstract

The activation of murine peritoneal macrophages by Ganoderma lucidum polysaccharides liposomes (GLPL) was investigated in vitro. After treatment with GLPL, the changes of the nitric oxide (NO) secretion and iNOS (inducible nitric oxide synthase) activity were evaluated. The results showed that NO production and iNOS activity of macrophages were enhanced compared to GLP and BL group. In addition, both the phagocytic activity and levels of cytokines IL-1β, TNF-α and IFN-γ were enhanced in the peritoneal macrophages of mice by stimulation of GLPL. The expression of the major histocompatibility complex class II molecule (MHC II) on the surface of peritoneal macrophages significantly increased. These indicated that GLPL could enhance the activation of peritoneal macrophages and their potential for use as a delivery system of GLP.

KEYWORDS:

Ganoderma lucidum polysaccharides liposome; MHC II; Peritoneal macrophages; Phagocytosis

PMID:
26529190
DOI:
10.1016/j.ijbiomac.2015.10.088
[Indexed for MEDLINE]

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