Format

Send to

Choose Destination
Methods. 2016 May 15;101:36-42. doi: 10.1016/j.ymeth.2015.10.014. Epub 2015 Oct 23.

Controlling transcription in human pluripotent stem cells using CRISPR-effectors.

Author information

1
Program in Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA.
2
Program in Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA. Electronic address: rene.maehr@umassmed.edu.

Abstract

The ability to manipulate transcription in human pluripotent stem cells (hPSCs) is fundamental for the discovery of key genes and mechanisms governing cellular state and differentiation. Recently developed CRISPR-effector systems provide a systematic approach to rapidly test gene function in mammalian cells, including hPSCs. In this review, we discuss recent advances in CRISPR-effector technologies that have been employed to control transcription through gene activation, gene repression, and epigenome engineering. We describe an application of CRISPR-effector mediated transcriptional regulation in hPSCs by targeting a synthetic promoter driving a GFP transgene, demonstrating the ease and effectiveness of CRISPR-effector mediated transcriptional regulation in hPSCs.

KEYWORDS:

CRISPR; Cas9; Human pluripotent stem cells; Transcriptional regulation; dCas9-effectors

PMID:
26525193
PMCID:
PMC4842159
DOI:
10.1016/j.ymeth.2015.10.014
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center