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Nat Biotechnol. 2015 Dec;33(12):1287-1292. doi: 10.1038/nbt.3386. Epub 2015 Nov 2.

GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell interactions.

Author information

1
Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
2
Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
3
The New York Stem Cell Foundation Research Institute, New York, New York, USA.
4
Mount Sinai Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
5
Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
6
Diabetes Obesity and Metabolism Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.

Abstract

There are numerous cell types with scarcely understood functions, whose interactions with the immune system are not well characterized. To facilitate their study, we generated a mouse bearing enhanced green fluorescent protein (EGFP)-specific CD8+ T cells. Transfer of the T cells into EGFP reporter animals can be used to kill EGFP-expressing cells, allowing selective depletion of desired cell types, or to interrogate T-cell interactions with specific populations. Using this system, we eliminate a rare EGFP-expressing cell type in the heart and demonstrate its role in cardiac function. We also show that naive T cells are recruited into the mouse brain by antigen-expressing microglia, providing evidence of an immune surveillance pathway in the central nervous system. The just EGFP death-inducing (Jedi) T cells enable visualization of a T-cell antigen. They also make it possible to utilize hundreds of existing EGFP-expressing mice, tumors, pathogens and other tools, to study T-cell interactions with many different cell types, to model disease states and to determine the functions of poorly characterized cell populations.

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