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Nat Methods. 2015 Dec;12(12):1129-31. doi: 10.1038/nmeth.3652. Epub 2015 Nov 2.

Thermal proteome profiling monitors ligand interactions with cellular membrane proteins.

Author information

1
Cellzome GmbH, Molecular Discovery Research, GlaxoSmithKline, Heidelberg, Germany.
2
Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

We extended thermal proteome profiling to detect transmembrane protein-small molecule interactions in cultured human cells. When we assessed the effects of detergents on ATP-binding profiles, we observed shifts in denaturation temperature for ATP-binding transmembrane proteins. We also observed cellular thermal shifts in pervanadate-induced T cell-receptor signaling, delineating the membrane target CD45 and components of the downstream pathway, and with drugs affecting the transmembrane transporters ATP1A1 and MDR1.

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PMID:
26524241
DOI:
10.1038/nmeth.3652
[Indexed for MEDLINE]

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