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Crit Rev Biochem Mol Biol. 2016;51(2):86-95. doi: 10.3109/10409238.2015.1103205. Epub 2015 Nov 2.

Cell-based screens and phenomics with fission yeast.

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a Research Department of Genetics , Evolution and Environment and UCL Institute of Healthy Ageing, University College London , London , UK.


Next-generation sequencing approaches have considerably advanced our understanding of genome function and regulation. However, the knowledge of gene function and complex cellular processes remains a challenge and bottleneck in biological research. Phenomics is a rapidly emerging area, which seeks to rigorously characterize all phenotypes associated with genes or gene variants. Such high-throughput phenotyping under different conditions can be a potent approach toward gene function. The fission yeast Schizosaccharomyces pombe (S. pombe) is a proven eukaryotic model organism that is increasingly used for genomewide screens and phenomic assays. In this review, we highlight current large-scale, cell-based approaches used with S. pombe, including computational colony-growth measurements, genetic interaction screens, parallel profiling using barcodes, microscopy-based cell profiling, metabolomic methods and transposon mutagenesis. These diverse methods are starting to offer rich insights into the relationship between genotypes and phenotypes.


Drug screen; functional profiling; metabolomics; microbial growth; mutant screen

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