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Cell. 2015 Nov 5;163(4):866-79. doi: 10.1016/j.cell.2015.10.017. Epub 2015 Oct 29.

Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation.

Author information

1
University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland.
2
U1006 INSERM, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 avenue de Luminy, 13009 Marseille, France.
3
University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland; Swiss National Centre for Competence in Research Programme Chemical Biology, 1211 Geneva, Switzerland; U1006 INSERM, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 avenue de Luminy, 13009 Marseille, France.
4
LPTMS,CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91405 Orsay, France.
5
U1006 INSERM, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 avenue de Luminy, 13009 Marseille, France. Electronic address: simon.scheuring@inserm.fr.
6
University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland; Swiss National Centre for Competence in Research Programme Chemical Biology, 1211 Geneva, Switzerland. Electronic address: aurelien.roux@unige.ch.

Abstract

ESCRT-III is required for lipid membrane remodeling in many cellular processes, from abscission to viral budding and multi-vesicular body biogenesis. However, how ESCRT-III polymerization generates membrane curvature remains debated. Here, we show that Snf7, the main component of ESCRT-III, polymerizes into spirals at the surface of lipid bilayers. When covering the entire membrane surface, these spirals stopped growing when densely packed: they had a polygonal shape, suggesting that lateral compression could deform them. We reasoned that Snf7 spirals could function as spiral springs. By measuring the polymerization energy and the rigidity of Snf7 filaments, we showed that they were deformed while growing in a confined area. Furthermore, we observed that the elastic expansion of compressed Snf7 spirals generated an area difference between the two sides of the membrane and thus curvature. This spring-like activity underlies the driving force by which ESCRT-III could mediate membrane deformation and fission.

Comment in

PMID:
26522593
PMCID:
PMC4644223
DOI:
10.1016/j.cell.2015.10.017
[Indexed for MEDLINE]
Free PMC Article

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