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New Phytol. 2016 Mar;209(4):1655-67. doi: 10.1111/nph.13710. Epub 2015 Nov 2.

The syntaxin protein (MoSyn8) mediates intracellular trafficking to regulate conidiogenesis and pathogenicity of rice blast fungus.

Qi Z1,2, Liu M1,2, Dong Y1,2, Zhu Q1,2, Li L1,2, Li B1,2, Yang J1,2, Li Y1,2, Ru Y1,2, Zhang H1,2, Zheng X1,2, Wang P3, Zhang Z1,2.

Author information

1
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.
2
Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
3
Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, LA, 70118, USA.

Abstract

Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) mediate cellular membrane fusion and intracellular vesicle trafficking in eukaryotic cells, and are critical in the growth and development of pathogenic fungi such as Magnaporthe oryzae which causes rice blast. Rice blast is thought to involve distinct SNARE-mediated transport and secretion of fungal effector proteins into the host to modulate rice immunity. We have previously characterized two SNARE proteins, secretory protein (MoSec22) and vesicle-associated membrane protein (MoVam7), as being important in cellular transport and pathogenicity. Here, we show that syntaxin 8 (MoSyn8), a Qc-SNARE protein homolog, also plays important roles in growth, conidiation, and pathogenicity. The MoSYN8 deletion mutant (∆Mosyn8) mutant exhibits defects in endocytosis and F-actin organization, appressorium turgor pressure generation, and host penetration. In addition, the ∆Mosyn8 mutant cannot elaborate biotrophic invasion of the susceptible rice host, or secrete avirulence factors Avr-Pia (corresponding to the rice resistance gene Pia) and Avrpiz-t (the cognate Avr gene for the resistance gene Piz-t) proteins. Our study of MoSyn8 advances our understanding of SNARE proteins in effector secretion which underlies the normal physiology and pathogenicity of M. oryzae, and it sheds new light on the mechanism of the blight disease caused by M. oryzae.

KEYWORDS:

Magnapother oryzae; Qc-SNARE; conidiogenesis; endocytosis; pathogenesis; secretion

PMID:
26522477
DOI:
10.1111/nph.13710
[Indexed for MEDLINE]
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