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Curr Protoc Protein Sci. 2015 Nov 2;82:10.7.1-16. doi: 10.1002/0471140864.ps1007s82.

Electroblotting from Polyacrylamide Gels.

Author information

1
The Wistar Institute, Philadelphia, Pennsylvania.

Abstract

Transferring proteins from polyacrylamide gels onto retentive membranes is now primarily used for immunoblotting. A second application that was quite common up to about a decade ago was electroblotting of proteins for N-terminal and internal sequencing using Edman chemistry. This unit contains procedures for electroblotting proteins from polyacrylamide gels onto a variety of membranes, including polyvinylidene difluoride (PVDF) and nitrocellulose. In addition to the commonly used tank or wet transfer system, protocols are provided for electroblotting using semidry and dry systems. This unit also describes procedures for eluting proteins from membranes using detergents or acidic extraction with organic solvents for specialized applications.

KEYWORDS:

SDS-PAGE gel transfer; electroblotting; electrotransfer; polyacrylamide gel transfer; protein transfer; western blotting

PMID:
26521711
PMCID:
PMC4663689
DOI:
10.1002/0471140864.ps1007s82
[Indexed for MEDLINE]
Free PMC Article

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