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J Photochem Photobiol B. 2015 Dec;153:344-51. doi: 10.1016/j.jphotobiol.2015.10.015. Epub 2015 Oct 20.

Photobiomodulation with 660-nm and 780-nm laser on activated J774 macrophage-like cells: Effect on M1 inflammatory markers.

Author information

1
Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho/UNINOVE, 249 Vergueiro Street, Liberdade, Sao Paulo, SP Zip Code 01504-001, Brazil; Department of Oral Pathology, Dental School, Universidade de Sao Paulo, 2227 Professor Lineu Prestes Avenue, Cidade Universitaria, Sao Paulo, SP Zip Code 05508-000, Brazil. Electronic address: kristianneporta@gmail.com.
2
Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho/UNINOVE, 249 Vergueiro Street, Liberdade, Sao Paulo, SP Zip Code 01504-001, Brazil. Electronic address: nadhiacosta@gmail.com.
3
Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho/UNINOVE, 249 Vergueiro Street, Liberdade, Sao Paulo, SP Zip Code 01504-001, Brazil; Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho/UNINOVE, 249 Vergueiro Street, Liberdade, Sao Paulo, SP Zip Code 01504-001, Brazil. Electronic address: raquel.mesquita@gmail.com.
4
Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho/UNINOVE, 249 Vergueiro Street, Liberdade, Sao Paulo, SP Zip Code 01504-001, Brazil. Electronic address: fatesi@uol.com.br.
5
Department of Oral Pathology, Dental School, Universidade de Sao Paulo, 2227 Professor Lineu Prestes Avenue, Cidade Universitaria, Sao Paulo, SP Zip Code 05508-000, Brazil. Electronic address: lilia_mail81@yahoo.com.br.
6
Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho/UNINOVE, 249 Vergueiro Street, Liberdade, Sao Paulo, SP Zip Code 01504-001, Brazil. Electronic address: agnelonevesalves@gmail.com.
7
Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho/UNINOVE, 249 Vergueiro Street, Liberdade, Sao Paulo, SP Zip Code 01504-001, Brazil. Electronic address: kaline.bsousa@gmail.com.
8
Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho/UNINOVE, 249 Vergueiro Street, Liberdade, Sao Paulo, SP Zip Code 01504-001, Brazil; Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho/UNINOVE, 249 Vergueiro Street, Liberdade, Sao Paulo, SP Zip Code 01504-001, Brazil. Electronic address: sandra.skb@gmail.com.
9
Wellman Center for Photomedicine, Massachusetts General Hospital, BAR414, 40 Blossom Street, Boston, MA 02114, United States; Department of Dermatology, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, United States; Harvard-MIT Division of Health Sciences and Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States. Electronic address: hamblin@helix.mgh.harvard.edu.
10
Department of Oral Pathology, Dental School, Universidade de Sao Paulo, 2227 Professor Lineu Prestes Avenue, Cidade Universitaria, Sao Paulo, SP Zip Code 05508-000, Brazil. Electronic address: fadnunes@usp.br.

Abstract

M1 profile macrophages exert a major influence on initial tissue repair process. Few days after the occurrence of injury, macrophages in the injured region exhibit a M2 profile, attenuate the effects of the M1 population, and stimulate the reconstruction of the damaged tissue. The different effects of macrophages in the healing process suggest that these cells could be the target of therapeutic interventions. Photobiomodulation has been used to accelerate tissue repair, but little is known regarding its effect on macrophages. In the present study, J774 macrophages were activated to simulate the M1 profile and irradiated with two different sets of laser parameters (780 nm, 70 mW, 2.6J/cm(2), 1.5s and 660 nm, 15 mW, 7.5 J/cm(2), 20s). IL-6, TNF-α, iNOS and COX-2 gene and protein expression were analyzed by RT-qPCR and ELISA. Both lasers were able to reduce TNF-α and iNOS expression, and TNF-α and COX-2 production, although the parameters used for 780 nm laser provided an additional decrease. 660 nm laser parameters resulted in an up-regulation of IL-6 expression and production. These findings imply a distinct, time-dependent modulation by the two different sets of laser parameters, suggesting that the best modulation may involve more than one combination of parameters.

KEYWORDS:

Cytokines; Inflammatory markers; J774 cells; LLLT; M1-activated macrophages; Photobiomodulation

PMID:
26519828
PMCID:
PMC4674369
DOI:
10.1016/j.jphotobiol.2015.10.015
[Indexed for MEDLINE]
Free PMC Article

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