Format

Send to

Choose Destination
Virology. 2015 Dec;486:272-83. doi: 10.1016/j.virol.2015.07.021. Epub 2015 Oct 27.

A novel insect-specific flavivirus replicates only in Aedes-derived cells and persists at high prevalence in wild Aedes vigilax populations in Sydney, Australia.

Author information

1
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Queensland, Australia. Electronic address: breeanna.mclean@uqconnect.edu.au.
2
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Queensland, Australia. Electronic address: j.peters2@uq.edu.au.
3
Medical Entomology, Marie Bashir Institute of Infectious Diseases and Biosecurity, The University of Sydney, NSW, Australia. Electronic address: Cameron.Webb@health.nsw.gov.au.
4
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Queensland, Australia. Electronic address: d.watterson@uq.edu.au.
5
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Queensland, Australia. Electronic address: Natalie.Prow@QIMRBerghofer.edu.au.
6
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Queensland, Australia. Electronic address: hongduyennguyenthi@gmail.com.
7
Virology, Public and Environmental Health, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia. Electronic address: Sonja.Hall-Mendelin@health.qld.gov.au.
8
Virology, Public and Environmental Health, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia. Electronic address: David.Warrilow@health.qld.gov.au.
9
School of Pathology and Laboratory Medicine, The University of Western Australia, Western Australia, Australia. Electronic address: cheryl.johansen@uwa.edu.au.
10
Virology, Public and Environmental Health, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia. Electronic address: Cassie.Jansen@health.qld.gov.au.
11
Virology, Public and Environmental Health, Forensic and Scientific Services, Department of Health, Queensland Government, Queensland, Australia. Electronic address: Andrew.VanDenHurk@health.qld.gov.au.
12
School of Biological Sciences, University of Queensland, Queensland, Australia; CSIRO Biosecurity Flagship, Dutton Park, Queensland, Australia. Electronic address: n.beebe@uq.edu.au.
13
MRC - University of Glasgow Centre for Virus Research, Glasgow, United Kingdom. Electronic address: Esther.Schnettler@glasgow.ac.uk.
14
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Queensland, Australia. Electronic address: rossbarnard@uq.edu.au.
15
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Queensland, Australia. Electronic address: roy.hall@uq.edu.au.

Abstract

To date, insect-specific flaviviruses (ISFs) have only been isolated from mosquitoes and increasing evidence suggests that ISFs may affect the transmission of pathogenic flaviviruses. To investigate the diversity and prevalence of ISFs in Australian mosquitoes, samples from various regions were screened for flaviviruses by ELISA and RT-PCR. Thirty-eight pools of Aedes vigilax from Sydney in 2007 yielded isolates of a novel flavivirus, named Parramatta River virus (PaRV). Sequencing of the viral RNA genome revealed it was closely related to Hanko virus with 62.3% nucleotide identity over the open reading frame. PaRV failed to grow in vertebrate cells, with only Aedes-derived mosquito cell lines permissive to replication, suggesting a narrow host range. 2014 collections revealed that PaRV had persisted in A. vigilax populations in Sydney, with 88% of pools positive. Further investigations into its mode of transmission and potential to influence vector competence of A. vigilax for pathogenic viruses are warranted.

KEYWORDS:

Aedes vigilax; Arbovirus; Coquillettidia xanthogaster; Insect-specific flavivirus; Mosquito; Palm Creek virus; Parramatta River virus; Virus discovery

PMID:
26519596
DOI:
10.1016/j.virol.2015.07.021
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center