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Methods Mol Biol. 2016;1369:293-308. doi: 10.1007/978-1-4939-3145-3_20.

Fission Yeast Cell Cycle Synchronization Methods.

Author information

1
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca, 37007, Salamanca, Spain.
2
Instituto de Investigación Biomédica de Salamanca (IBSAL), 37007, Salamanca, Spain.
3
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca, 37007, Salamanca, Spain. smo@usal.es.
4
Instituto de Investigación Biomédica de Salamanca (IBSAL), 37007, Salamanca, Spain. smo@usal.es.

Abstract

Fission yeast cells can be synchronized by cell cycle arrest and release or by size selection. Cell cycle arrest synchronization is based on the block and release of temperature-sensitive cell cycle mutants or treatment with drugs. The most widely used approaches are cdc10-129 for G1; hydroxyurea (HU) for early S-phase; cdc25-22 for G2, and nda3-KM311 for mitosis. Cells can also be synchronized by size selection using centrifugal elutriation or a lactose gradient. Here we describe the methods most commonly used to synchronize fission yeast cells.

KEYWORDS:

Block and release; Centrifugal elutriation; Fission yeast; G1-phase; G2-phase; HU; Lactose gradient; Mitosis; S-phase; Schizosaccharomyces pombe; Synchronization; cdc10-129; cdc25-22; nda3-KM311

PMID:
26519320
DOI:
10.1007/978-1-4939-3145-3_20
[Indexed for MEDLINE]

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