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J Biol Chem. 2015 Dec 18;290(51):30342-50. doi: 10.1074/jbc.M115.691857. Epub 2015 Oct 30.

miR-346 Up-regulates Argonaute 2 (AGO2) Protein Expression to Augment the Activity of Other MicroRNAs (miRNAs) and Contributes to Cervical Cancer Cell Malignancy.

Author information

1
From the Tianjin Life Science Research Center, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
2
From the Tianjin Life Science Research Center, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China htang2002@yahoo.com.

Abstract

MicroRNAs (miRNAs) are a class of post-transcriptional regulators of gene expression, and AGO2 is essential for miRNA activity. In this study, we focused on the regulation of AGO2 by miR-346 and the consequences in cervical cancer cells. miR-346 enhanced the expression of AGO2, resulting in the increased activity of other miRNAs and contributing to the malignancy of HeLa cells. GRSF1 participated in the regulation of AGO2 by miR-346, and the middle sequence of miR-346 was vital for the synergy effect of miR-346 and GRSF1. We determined that miR-346 promoted the migration and invasion of HeLa cells. In summary, we are the first to report that AGO2 is regulated positively by miRNA and that GRSF1 participates in the miRNA pathway.

KEYWORDS:

AGO2; GRSF1; eukaryotic initiation factor 2 (eIF2); gene activation; gene expression; gene regulation; miR-346; microRNA (miRNA); microRNA mechanism

PMID:
26518874
PMCID:
PMC4683258
DOI:
10.1074/jbc.M115.691857
[Indexed for MEDLINE]
Free PMC Article

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