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BMC Neurosci. 2015 Oct 30;16:72. doi: 10.1186/s12868-015-0202-2.

High-fat diet-induced obesity exacerbates kainic acid-induced hippocampal cell death.

Author information

1
Department of Neurosurgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, 15, Jinju-daero 816 Beon-gil, Jinju-si, Gyeongnam, Republic of Korea. ns4793@hanmail.net.
2
Department of Anatomy and Convergence Medical Science, Institute of Health Sciences, Gyeongsang National University School of Medicine, 15, Jinju-daero 816 Beon-gil, Jinju-si, Gyeongnam, Republic of Korea. amethyst-22@nate.com.
3
Department of Anatomy and Convergence Medical Science, Institute of Health Sciences, Gyeongsang National University School of Medicine, 15, Jinju-daero 816 Beon-gil, Jinju-si, Gyeongnam, Republic of Korea. enemy1725@nate.com.
4
Department of Anatomy and Convergence Medical Science, Institute of Health Sciences, Gyeongsang National University School of Medicine, 15, Jinju-daero 816 Beon-gil, Jinju-si, Gyeongnam, Republic of Korea. hwajin1@gmail.com.
5
Department of Neurosurgery, Pusan National University School of Medicine, 179 Gudeok-ro, Seo-gu, Busan, Republic of Korea. chwachoi@pusan.ac.kr.
6
Department of Anatomy and Convergence Medical Science, Institute of Health Sciences, Gyeongsang National University School of Medicine, 15, Jinju-daero 816 Beon-gil, Jinju-si, Gyeongnam, Republic of Korea. anaroh@gnu.ac.kr.

Abstract

BACKGROUND:

Obesity has deleterious effects on the brain, and metabolic dysfunction may exacerbate the outcomes of seizures and brain injuries. However, it is unclear whether obesity affects excitotoxicity-induced neuronal cell death. The purpose of this study was to investigate the effects of a high-fat diet (HFD) on neuroinflammation and oxidative stress in the hippocampus of kainic acid (KA)-treated mice.

RESULTS:

Mice were fed with a HFD or normal diet for 8 weeks and then received a systemic injection of KA. HFD-fed mice showed hypercholesterolemia, insulin resistance, and hepatic steatosis. HFD-fed mice showed greater susceptibility to KA-induced seizures, an increased number of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells, neuroinflammation, and oxidative stress. Furthermore, we found that KA treatment increased HFD-induced calpain1, nuclear factor E2-related factor 2, and heme oxygenase-1 expression in the hippocampus.

CONCLUSIONS:

These findings imply that complex mechanisms affected by obesity-induced systemic inflammation, neuroinflammation, ER stress, calcium overload, and oxidative stress may contribute to neuronal death after brain injury.

PMID:
26518260
PMCID:
PMC4628384
DOI:
10.1186/s12868-015-0202-2
[Indexed for MEDLINE]
Free PMC Article

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