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BMC Genomics. 2015 Oct 30;16:885. doi: 10.1186/s12864-015-2095-y.

The epigenetic processes of meiosis in male mice are broadly affected by the widely used herbicide atrazine.

Author information

1
Inserm U1085 IRSET, 263 Avenue du Général Leclerc, 35042, Rennes, France. aurore.gely-pernot@univ-rennes1.fr.
2
Inserm U1085 IRSET, 263 Avenue du Général Leclerc, 35042, Rennes, France. chunxiang.hao@univ-rennes1.fr.
3
Inserm U1085 IRSET, 263 Avenue du Général Leclerc, 35042, Rennes, France. emmanuelle.becker@univ-rennes1.fr.
4
Inserm U1085 IRSET, 263 Avenue du Général Leclerc, 35042, Rennes, France. istupa@pbf.hr.
5
Present address: University of Zagreb, Faculty of Food Technology and Biotechnology, 10000, Zagreb, Croatia. istupa@pbf.hr.
6
Inserm U1085 IRSET, 263 Avenue du Général Leclerc, 35042, Rennes, France. christine.kervarrec@inserm.fr.
7
Inserm U1085 IRSET, 263 Avenue du Général Leclerc, 35042, Rennes, France. frederic.chalmel@inserm.fr.
8
Inserm U1085 IRSET, 263 Avenue du Général Leclerc, 35042, Rennes, France. michael.primig@inserm.fr.
9
Inserm U1085 IRSET, 263 Avenue du Général Leclerc, 35042, Rennes, France. bernard.jegou@inserm.fr.
10
EHESP, Avenue du Professeur Léon-Bernard, 35043, Rennes, France. bernard.jegou@inserm.fr.
11
Inserm U1085 IRSET, 263 Avenue du Général Leclerc, 35042, Rennes, France. fatima.smagulova@inserm.fr.

Abstract

BACKGROUND:

Environmental factors such as pesticides can cause phenotypic changes in various organisms, including mammals. We studied the effects of the widely used herbicide atrazine (ATZ) on meiosis, a key step of gametogenesis, in male mice.

METHODS:

Gene expression pattern was analysed by Gene-Chip array. Genome-wide mapping of H3K4me3 marks distribution was done by ChIP-sequencing of testis tissue using Illumina technologies. RT-qPCR was used to validate differentially expressed genes or differential peaks.

RESULTS:

We demonstrate that exposure to ATZ reduces testosterone levels and the number of spermatozoa in the epididymis and delays meiosis. Using Gene-Chip and ChIP-Seq analysis of H3K4me3 marks, we found that a broad range of cellular functions, including GTPase activity, mitochondrial function and steroid-hormone metabolism, are affected by ATZ. Furthermore, treated mice display enriched histone H3K4me3 marks in regions of strong recombination (double-strand break sites), within very large genes and reduced marks in the pseudoautosomal region of X chromosome.

CONCLUSIONS:

Our data demonstrate that atrazine exposure interferes with normal meiosis, which affects spermatozoa production.

PMID:
26518232
PMCID:
PMC4628360
DOI:
10.1186/s12864-015-2095-y
[Indexed for MEDLINE]
Free PMC Article

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