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Curr Opin Chem Biol. 2015 Dec;29:72-8. doi: 10.1016/j.cbpa.2015.10.001. Epub 2015 Oct 24.

How specific is CRISPR/Cas9 really?

Author information

1
Genome Center, Department of Biochemistry and Molecular Medicine, University of California, Davis, CA 95616, USA.
2
Genome Center, Department of Biochemistry and Molecular Medicine, University of California, Davis, CA 95616, USA. Electronic address: djsegal@ucdavis.edu.

Abstract

The specificity of RNA-guided nucleases has gathered considerable interest as they become broadly applied to basic research and therapeutic development. Reports of the simple generation of animal models and genome engineering of cells raised questions about targeting precision. Conflicting early reports led the field to believe that CRISPR/Cas9 system was promiscuous, leading to a variety of strategies for improving specificity and increasingly sensitive methods to detect off-target events. However, other studies have suggested that CRISPR/Cas9 is a highly specific genome-editing tool. This review will focus on deciphering and interpreting these seemingly opposing claims.

PMID:
26517564
PMCID:
PMC4684463
DOI:
10.1016/j.cbpa.2015.10.001
[Indexed for MEDLINE]
Free PMC Article

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