Format

Send to

Choose Destination
See comment in PubMed Commons below
Viruses. 2015 Oct 23;7(10):5508-24. doi: 10.3390/v7102887.

Identifying Recent HIV Infections: From Serological Assays to Genomics.

Author information

  • 1Division of Medical Virology, Stellenbosch University, Tygerberg 7505, South Africa. sikhulilemoyo@gmail.com.
  • 2Botswana-Harvard AIDS Institute Partnership, Private Bag BO320, Gaborone, Botswana. sikhulilemoyo@gmail.com.
  • 3Africa Centre for Health and Population Studies, Doris Duke Medical Research Centre, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban 4041, South Africa. ewilkinson83@gmail.com.
  • 4Botswana-Harvard AIDS Institute Partnership, Private Bag BO320, Gaborone, Botswana. vnovi@hsph.harvard.edu.
  • 5Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA. vnovi@hsph.harvard.edu.
  • 6Africa Centre for Health and Population Studies, Doris Duke Medical Research Centre, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban 4041, South Africa. vando026@umn.edu.
  • 7Botswana-Harvard AIDS Institute Partnership, Private Bag BO320, Gaborone, Botswana. sgaseitsiwe@bhp.org.bw.
  • 8Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA. sgaseitsiwe@bhp.org.bw.
  • 9Botswana-Harvard AIDS Institute Partnership, Private Bag BO320, Gaborone, Botswana. messex@hsph.harvard.edu.
  • 10Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA. messex@hsph.harvard.edu.
  • 11Division of Medical Virology, Stellenbosch University, Tygerberg 7505, South Africa. susanen@sun.ac.za.
  • 12National Health Laboratory Services (NHLS), Tygerberg Coastal, Johannesburg 2001, South Africa. susanen@sun.ac.za.
  • 13Africa Centre for Health and Population Studies, Doris Duke Medical Research Centre, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban 4041, South Africa. tdeoliveira@africacentre.ac.za.
  • 14Research Department of Infection, University College London, London WC1E 6BT, UK. tdeoliveira@africacentre.ac.za.
  • 15College of Health Sciences, University of KwaZulu-Natal, Durban 4041, South Africa. tdeoliveira@africacentre.ac.za.

Abstract

In this paper, we review serological and molecular based methods to identify HIV infection recency. The accurate identification of recent HIV infection continues to be an important research area and has implications for HIV prevention and treatment interventions. Longitudinal cohorts that follow HIV negative individuals over time are the current gold standard approach, but they are logistically challenging, time consuming and an expensive enterprise. Methods that utilize cross-sectional testing and biomarker information have become an affordable alternative to the longitudinal approach. These methods use well-characterized biological makers to differentiate between recent and established HIV infections. However, recent results have identified a number of limitations in serological based assays that are sensitive to the variability in immune responses modulated by HIV subtypes, viral load and antiretroviral therapy. Molecular methods that explore the dynamics between the timing of infection and viral evolution are now emerging as a promising approach. The combination of serological and molecular methods may provide a good solution to identify recent HIV infection in cross-sectional data. As part of this review, we present the advantages and limitations of serological and molecular based methods and their potential complementary role for the identification of HIV infection recency.

KEYWORDS:

molecular-based assays; recent HIV infection; serology-based assays; viral diversity

PMID:
26512688
PMCID:
PMC4632395
DOI:
10.3390/v7102887
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
    Loading ...
    Support Center