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Proc Natl Acad Sci U S A. 2015 Nov 10;112(45):14096-101. doi: 10.1073/pnas.1512865112. Epub 2015 Oct 28.

KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1.

Author information

1
Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany;
2
Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany; Instituto de Agrobiotecnología del Litoral, Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Técnicas, 3000 Santa Fe, Argentina pablomanavella@ial.santafe-conicet.gov.ar.

Abstract

The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. We have identified the Arabidopsis thaliana K homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples of rcf3 mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). These interactions are essential to regulate the phosphorylation status, and thus the activity, of the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) cofactor HYPONASTIC LEAVES1 (HYL1).

KEYWORDS:

Arabidopsis thaliana; HYL1; gene silencing; micro RNA biogenesis; phosphorylation

PMID:
26512101
PMCID:
PMC4653147
DOI:
10.1073/pnas.1512865112
[Indexed for MEDLINE]
Free PMC Article

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