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In Vitro Cell Dev Biol Anim. 2016 Feb;52(2):144-8. doi: 10.1007/s11626-015-9964-1. Epub 2015 Oct 28.

Caspases and osteogenic markers--in vitro screening of inhibition impact.

Author information

1
Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic.
2
Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
3
Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
4
Institute of Analytical Chemistry, v.v.i., Brno, Czech Republic.
5
Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic. matalova@iach.cz.
6
Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. matalova@iach.cz.

Abstract

Caspases, well-known players in apoptosis or inflammation, appear to have roles also in other processes such as cell differentiation. Caspase-3, in particular, was recently demonstrated to have non-apoptotic functions in osteogenesis. However, the molecular pathways involved are not yet known. Therefore, we used osteogenic PCR arrays to provide a comprehensive screening of possible interactions of caspases in general and specifically of caspase-3 in osteogenic networks. Embryonic micromass cultures derived from mouse forelimbs were established and pharmacological fluoromethylketone (FMK) inhibitors applied. Alterations were observed in expression of several genes after caspase inhibition (Bmp1, Bmp5, Bmp6, Col10a1, Col2a1, Comp, Egf, Fgfr2, Gli1, Igf1, Nog, Phex, Sox9, Spp1). The list suggests molecular interactions of caspases and osteogenic molecules and creates a background for further temporospatial and functional studies.

KEYWORDS:

Caspase-3; Caspases; Chondrogenesis; Gene expression; Osteogenesis

PMID:
26512002
DOI:
10.1007/s11626-015-9964-1
[Indexed for MEDLINE]

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