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Innate Immun. 2016 Jan;22(1):9-20. doi: 10.1177/1753425915609973. Epub 2015 Oct 28.

Innate immunity of surfactant proteins A and D in urinary tract infection with uropathogenic Escherichia coli.

Author information

1
Department of Surgery, SUNY Upstate Medical University, Syracuse, NY, USA Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, China.
2
Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, China.
3
Department of Surgery, SUNY Upstate Medical University, Syracuse, NY, USA.
4
Department of Biological Sciences, SUNY Cortland, Cortland, NY, USA.
5
Department of Pediatrics and the Cardiovascular Research Institute, University of California, San Francisco, CA, USA.
6
Division of Neonatology, Department of Pediatrics, Children's Hospital, University of California Davis Medical Center, Davis, CA, USA.
7
Department of Surgery, SUNY Upstate Medical University, Syracuse, NY, USA wangg@upstate.edu ghxding@gmail.com.

Abstract

To investigate the effects of surfactant proteins A and D (SP-A and SP-D, respectively) in urinary tract infection (UTI), SP-A and SP-D double knockout (SP-A/D KO) and wild type (WT) C57BL/6 female mice were infected with uropathogenic Escherichia coli by intravesical inoculation. Compared with WT mice SP-A/D KO mice showed increased susceptibility to UTI, as evidenced by higher bacterial CFU, more infiltrating neutrophils and severe pathological changes. Keratinocyte-derived chemokine increased in the kidney of WT mice but not in SP-A/D KO mice 24 h post-infection. Compared with control, the level of IL-17 was elevated in the kidney of infected WT and SP-A/D KO mice and the level of IL-17 was higher in the infected SP-A/D KO mice than in infected WT mice 24 and 48 h post-infection. The basal level of p38 MAPK phosphorylation in SP-A/D KO mice was higher than in WT mice. The phosphorylated p38 level was elevated in the kidney of WT mice post infection but not in SP-A/D KO mice. Furthermore, in vitro growth of uropathogenic E. coli was inhibited by SP-A and SP-D. We conclude that SP-A and SP-D function as mediators of innate immunity by inhibiting bacterial growth and modulating renal inflammation in part by regulating p38 MAPK-related pathway in murine UTI.

KEYWORDS:

Innate immunity; surfactant protein A; surfactant protein D; urinary tract infection; uropathogenic Escherichia coli

PMID:
26511057
PMCID:
PMC4679646
DOI:
10.1177/1753425915609973
[Indexed for MEDLINE]
Free PMC Article

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