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Pharm Biol. 2016;54(4):674-85. doi: 10.3109/13880209.2015.1072565. Epub 2015 Oct 29.

Implication of thymoquinone as a remedy for polycystic ovary in rat.

Author information

1
a Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University , New Delhi , India and.
2
b Reproductive Toxicology Laboratory, Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia , New Delhi , India.

Abstract

CONTEXT:

Thymoquinone (TQ), an active component of Nigella sativa L. (Ranunculaceae), possesses anti-inflammatory and anti-oxidative properties. Polycystic ovary syndrome exhibits chronic inflammatory behavior, thus might involve nuclear factor kappa B (NF-κB) signaling and related molecular factors.

OBJECTIVE:

The objective of the present study is to investigate and validate the effect of TQ in polycystic ovary (PCO) rat.

MATERIALS AND METHODS:

To validate the effect of TQ (1 µM/ml), NF-κB activation, COX2 (cyclooxygenase-2) expression and reactive oxygen species (ROS) induction were studied in the KK1 cell line. To evaluate the effect of TQ (2 mg/200 µl olive oil/rat; sc) with an in vivo system, ovulation rate, levels of key ovulation mediators, and ovarian gelatinases activity were compared in superovulated, PCO, and RU486 + TQ-treated Wistar rats.

RESULTS:

In vitro studies showed that NF-κB nuclear translocation, COX2, and ROS expression were repressed via TQ supplementation in RU486-treated KK1 cells. Pretreatment of TQ in the PCO rat model induced significant restoration of normal physio-molecular behavior of ovary, such as reduced cysts formation, increased ovulation rate, and normalization of key ovarian factors [like TNF-α-stimulated gene/protein 6, hyaluronan, hyaluronan-binding protein 1, COX2, matrix metalloproteinases (membrane type 1-matrix metalloproteinase, MMP9 and MMP2)], tissue inhibitor of metalloproteinases (TIMP-1 and TIMP-2), and gelatinases (like MMP9 and -2) activity during follicular maturation.

DISCUSSION AND CONCLUSION:

Overall, most of the above molecular changes are regulated via NF-κB pathway, thus TQ, due to its modulatory effect on the NF-κB signaling, could elevate normal ovarian phenotype and physiological function in the PCO model, indicating its remarkable potential as a remedy for rat PCO.

KEYWORDS:

COX2; NF-κB; hyaluronan-binding protein 1; matrix metalloproteinase

PMID:
26510692
DOI:
10.3109/13880209.2015.1072565
[Indexed for MEDLINE]

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