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Ann Surg Oncol. 2016 Feb;23(2):494-502. doi: 10.1245/s10434-015-4888-2. Epub 2015 Oct 27.

Inflammatory and Non-inflammatory Breast Cancer: A Potential Role for Detection of Multiple Viral DNAs in Disease Progression.

Author information

1
Department of General Surgery, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
2
Cancer Biology Research Lab, Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt. Hossam_viro2010@yahoo.com.
3
Cancer Biology Research Lab, Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.
4
Department of Pathology, National Cancer Institute, Cairo University, Giza, Egypt.
5
Department of Microbiology, School of Medicine, New York University, New York, NY, USA.

Abstract

BACKGROUND:

Inflammatory breast cancer (IBC) is the most lethal form of breast cancer. Multiple viral infections in IBC tissues were found to be associated with disease pathogenesis.

OBJECTIVE:

The aim of the present study was to correlate the incidence of viral DNA with breast cancer progression.

MATERIALS AND METHODS:

Overall, 135 women diagnosed with breast cancer were enrolled in this study. Using polymerase chain reaction and sequencing assays, we determined the incidence of human papillomavirus types 16 and 18 (HPV-16 and -18), human cytomegalovirus (HCMV), Epstein-Barr virus, human herpes simplex virus type 1 and 2, and human herpes virus type 8 (HHV-8) in breast carcinoma tissue biopsies. We also assessed the expression of the cell proliferation marker Ki-67 by immunohistochemistry in association with the incidence of viral DNA.

RESULTS:

HCMV and HPV-16 were the most detected viral DNAs in breast carcinoma tissues; however, the frequency of HCMV and HHV-8 DNA were significantly higher in IBC than non-IBC tissues. Moreover, the prevalence of multiple viral DNAs was higher in IBC than non-IBC tissues. The incidence of multiple viral DNAs positively correlates with tumor size and number of metastatic lymph nodes in both non-IBC and IBC patients. The expression of Ki-67 was found to be significantly higher in both non-IBC and IBC tissues in which multiple viral DNAs were detected.

CONCLUSIONS:

The incidence of multiple viral DNAs in IBC tissues was higher compared with non-IBC tissues. The present results suggest the possibility of a functional relationship between the presence of multiple viral DNAs and disease pathogenesis.

PMID:
26508152
DOI:
10.1245/s10434-015-4888-2
[Indexed for MEDLINE]

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