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Nat Rev Mol Cell Biol. 2015 Dec;16(12):753-61. doi: 10.1038/nrm4080. Epub 2015 Oct 28.

The systematic analysis of protein-lipid interactions comes of age.

Author information

1
Institute for Molecular Infection Biology and Core Unit Systems Medicine, University of Würzburg, Josef-Schneider-Strasse 2, D-97080 Würzburg, Germany.
2
European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
3
European Molecular Biology Laboratory (EMBL), Structural and Computational Biology Unit and Molecular Medicine Partnership Unit, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

Abstract

Lipids tailor membrane identities and function as molecular hubs in all cellular processes. However, the ways in which lipids modulate protein function and structure are poorly understood and still require systematic investigation. In this Innovation article, we summarize pioneering technologies, including lipid-overlay assays, lipid pull-down assays, affinity-purification lipidomics and the liposome microarray-based assay (LiMA), that will enable protein-lipid interactions to be deciphered on a systems level. We discuss how these technologies can be applied to the charting of system-wide networks and to the development of new pharmaceutical strategies.

PMID:
26507169
DOI:
10.1038/nrm4080
[Indexed for MEDLINE]

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