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J Biomed Mater Res B Appl Biomater. 2017 Feb;105(2):291-306. doi: 10.1002/jbm.b.33556. Epub 2015 Oct 27.

Preparation and characterization of a biologic scaffold and hydrogel derived from colonic mucosa.

Author information

1
McGowan Institute for Regenerative Medicine, Pittsburgh, Pennsylvania, 15219.
2
Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, 15213.
3
Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania, 15213.
4
Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, 15219.

Abstract

Gastrointestinal pathologies, injuries, and defects affect millions of individuals each year. While there are diverse treatment options for these individuals, no ideal solution exists. The repair or replacement of gastrointestinal tissue, therefore, represents a large unmet clinical need. Biomaterials derived from extracellular matrix (ECM) scaffolds have been effectively used to repair or replace numerous tissues throughout the body in both preclinical and clinical studies. Such scaffolds are prepared from decellularized tissues, and the biochemical, structural, and biologic properties vary depending upon the source tissue from which the ECM is derived. Given the potential benefit of a site-specific ECM scaffold for some applications, the objective of this study was to prepare, characterize, and determine the in vitro and in vivo cell response to ECM derived from porcine colon. Results of this study show that porcine colon can be effectively decellularized while retaining biochemical and structural constituents of the source tissue. Two forms of colonic ECM, scaffold and hydrogel, were shown to be cell friendly and facilitate the polarization of macrophages toward an M2 phenotype both in vitro and in vivo.

KEYWORDS:

extracellular matrix; hydrogel; regenerative medicine; scaffolds

PMID:
26506408
DOI:
10.1002/jbm.b.33556
[Indexed for MEDLINE]

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