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Molecules. 2015 Oct 22;20(10):19252-62. doi: 10.3390/molecules201019252.

Cytotoxicity of Triterpenes from Green Walnut Husks of Juglans mandshurica Maxim in HepG-2 Cancer Cells.

Author information

1
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. zhouyuanyuan1998@163.com.
2
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. ybywater@163.com.
3
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. liu_zhao_xi@163.com.
4
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. jiangyanqiu219@163.com.
5
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. ZYC19901014@126.com.
6
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. fl0451@outlook.com.
7
College of Adult Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China. wangxiaolicando2000@126.com.
8
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. hxkuang@hotmail.com.

Abstract

Among the classes of identified natural products, triterpenoids, one of the largest families, have been studied extensively for their diverse structures and variety of biological activities, including antitumor effects. In the present study, a phytochemical study of the green walnut husks of Juglans mandshurica Maxim led to the isolation of a new dammarane triterpene, 12β, 20(R), 24(R)-trihydroxydammar-25-en-3-one (6), together with sixteen known compounds, chiefly from chloroform and ethyl acetate extracts. According to their structural characteristics, these compounds were divided into dammarane-type, oleanane- and ursane-type. Dammarane-type triterpenoids were isolated for the first time from the Juglans genus. As part of our continuing search for biologically active compounds from this plant, all of these compounds were also evaluated for their cytotoxic activities against the growth of human cancer cells lines HepG-2 by the MTT assay. The results were shown that 20(S)-protopanaxadiol, 2α,3β,23-trihydroxyolean-12-en-28-oic acid and 2α,3β,23-trihydroxyurs-12-en-28-oic acid exhibited better cytotoxicity in vitro with IC50 values of 10.32±1.13, 16.13±3.83, 15.97±2.47 μM, respectively. Preliminary structure-activity relationships for these compounds were discussed.

KEYWORDS:

Juglans mandshurica Maxim; cytotoxic activity; green walnut husks; structure-activity relationships; triterpenoid aglycones

PMID:
26506336
PMCID:
PMC6331897
DOI:
10.3390/molecules201019252
[Indexed for MEDLINE]
Free PMC Article

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