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J Microbiol Methods. 2015 Dec;119:203-5. doi: 10.1016/j.mimet.2015.10.015. Epub 2015 Oct 23.

Extended-spectrum β-lactamase (ESBL) detection directly from urine samples with the rapid isothermal amplification-based eazyplex® SuperBug CRE assay: Proof of concept.

Author information

1
Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland. Electronic address: vladimira.hinic@usb.ch.
2
Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland.

Abstract

A commercially available assay (eazyplex® SuperBug CRE) detecting the most common carbapenemase and ESBL types was evaluated directly on 50 urine samples. Eazyplex® correctly detected ESBL-encoding genes in all 30 urine samples with confirmed ESBL production (sensitivity 100%). Two specimens showed invalid and one specimen false-positive results (specificity 97.9%).

KEYWORDS:

ESBL; Isothermal amplification; Proof of concept; Rapid detection; Urine samples

PMID:
26506282
DOI:
10.1016/j.mimet.2015.10.015
[Indexed for MEDLINE]

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