Format

Send to

Choose Destination
Cancer Cytopathol. 2016 Apr;124(4):241-53. doi: 10.1002/cncy.21639. Epub 2015 Oct 27.

Massively parallel DNA sequencing from routinely processed cytological smears.

Author information

1
Gustave Roussy, Villejuif, France.
2
Unité Mixte de Recherche 981, INSERM, Villejuif, France.
3
Genopole Campus 1, IntegraGen SA, Evry, France.
4
Genomic Platform, Translational Research Department, Institut Curie, Paris, France.
5
Analyse Moléculaire, Modélisation et Imagerie de la maladie Cancéreuse (AMMICa) CNRS Unité Mixte de Service 3655, INSERM US23, Paris Sud University), Translational Research Laboratory, Gustave Roussy, Villejuif, France.
6
Unit 1009, INSERM, Villejuif, France.
7
Department of Biopathology, Gustave Roussy, Villejuif, France.

Abstract

BACKGROUND:

Data generated by next-generation sequencing technologies have a pivotal role in precision medicine. These high-throughput techniques are preferentially performed on fresh tissue, but there is an increasing need for protocols adapted to materials derived from formalin-fixed, paraffin-embedded tissue and cytology specimens.

METHODS:

The aim of this work was to show that cytological material collected from archival smears processed for routine diagnoses could be used for massively parallel sequencing and array-based genomic analysis for further studies.

RESULTS:

As a proof of concept, data obtained from May-Grünwald Giemsa- and Diff-Quik-stained archival smears were shown to be in keeping with those obtained from matched frozen controls.

CONCLUSIONS:

The quality of DNA extracted from routinely processed smears is compatible with the multitargeted sequencing of a large series of genes of interest with methods such as array-based genomic analysis and whole-exome sequencing.

KEYWORDS:

cytological smear; macrodissection; next-generation sequencing; precision medicine; single-nucleotide polymorphism array; whole-exome sequencing

PMID:
26505273
DOI:
10.1002/cncy.21639
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center