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Mol Nutr Food Res. 2016 Feb;60(2):358-68. doi: 10.1002/mnfr.201500537. Epub 2015 Oct 26.

Fish protein increases circulating levels of trimethylamine-N-oxide and accelerates aortic lesion formation in apoE null mice.

Author information

1
Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany.
2
Institute of Human Nutrition and Food Science, University of Kiel, Kiel, Germany.

Abstract

SCOPE:

The protective effect of fish consumption on the cardiovascular system has primarily been ascribed to n-3 fatty acids, but data investigating the vascular effects of fish protein consumption are scarce. This study aimed to investigate the vascular impact of fish protein in a mouse model of atherosclerosis.

METHODS AND RESULTS:

Male apoE null mice were fed a Western diet containing 20% fish (turbot) protein, casein, or soy protein, for 16 wk. Morphometric analysis of the aorta revealed that the atherosclerotic plaque area of fish protein fed mice was twofold larger than that in casein- or soy protein-fed mice. The percentage area of calcification deposits in plaques of fish protein fed mice was higher (7.57%) than that in casein-fed (2.86%) or soy protein-fed (3.46%) mice, and fish protein fed mice exhibited higher plaque expression of CD68, CD36, and IL-6 than the other two groups. Fish protein intake was accompanied by increased serum concentrations of trimethylamine-N-oxide (7.03 ± 2.83 μmol/L), as compared with casein (0.92 ± 0.46 μmol/L) and soy protein (1.32 ± 0.54 μmol/L) intake.

CONCLUSION:

The present data indicate adverse effects of fish protein on the vascular system, which could be attributable to the high serum trimethylamine-N-oxide concentrations in these mice.

KEYWORDS:

Aortic lesion; Fish protein; Inflammation; Mice; Trimethylamine-N-oxide

PMID:
26502377
DOI:
10.1002/mnfr.201500537
[Indexed for MEDLINE]

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