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J Biol Chem. 2015 Dec 11;290(50):29993-30005. doi: 10.1074/jbc.M115.670018. Epub 2015 Oct 23.

Functional Characterization of Monomeric GTPase Rab1 in the Secretory Pathway of Leishmania.

Author information

1
From the National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India and.
2
the Institute of Microbial Technology, Chandigarh 160036, India.
3
From the National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India and amitabha@nii.res.in.

Abstract

Leishmania secretes a large number of its effectors to the extracellular milieu. However, regulation of the secretory pathway in Leishmania is not well characterized. Here, we report the cloning, expression, and characterization of the Rab1 homologue from Leishmania. We have found that LdRab1 localizes in Golgi in Leishmania. To understand the role of LdRab1 in the secretory pathway of Leishmania, we have generated transgenic parasites overexpressing GFP-LdRab1:WT, GFP-LdRab1:Q67L (a GTPase-deficient dominant positive mutant of Rab1), and GFP-LdRab1:S22N (a GDP-locked dominant negative mutant of Rab1). Surprisingly, our results have shown that overexpression of GFP-LdRab1:Q67L or GFP-LdRab1:S22N does not disrupt the trafficking and localization of hemoglobin receptor in Leishmania. To determine whether the Rab1-dependent secretory pathway is conserved in parasites, we have analyzed the role of LdRab1 in the secretion of secretory acid phosphatase and Ldgp63 in Leishmania. Our results have shown that overexpression of GFP-LdRab1:Q67L or GFP-LdRab1:S22N significantly inhibits the secretion of secretory acid phosphatase by Leishmania. We have also found that overexpression of GFP-LdRab1:Q67L or GFP-LdRab1:S22N retains RFP-Ldgp63 in Golgi and blocks the secretion of Ldgp63, whereas the trafficking of RFP-Ldgp63 in GFP-LdRab1:WT-expressing cells is unaltered in comparison with control cells. Taken together, our results have shown that the Rab1-regulated secretory pathway is well conserved, and hemoglobin receptor trafficking follows an Rab1-independent secretory pathway in Leishmania.

KEYWORDS:

Leishmania; Rab; Rab1; exocytosis; hemoglobin receptor; protein secretion; secretory pathway; trafficking

PMID:
26499792
PMCID:
PMC4706007
DOI:
10.1074/jbc.M115.670018
[Indexed for MEDLINE]
Free PMC Article

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