Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2015 Dec 4-11;468(1-2):343-8. doi: 10.1016/j.bbrc.2015.10.097. Epub 2015 Oct 22.

Macrophage-derived microvesicles promote proliferation and migration of Schwann cell on peripheral nerve repair.

Author information

1
Department of Orthopaedic Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China. Electronic address: zhchuansy@163.com.
2
Department of Orthopaedic Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China.

Abstract

BACKGROUND:

Macrophages have been implicated in peripheral nerve regeneration. However, whether macrophages-derived microvesicles (MVs) are involved in this process remains unknown. In the present study, the effects of macrophages-derived MVs on proliferation and migration of Schwann cells (SCs) were evaluated in both in vitro and in vivo.

METHODS:

Human monocytic leukaemia cell line (THP-1) was successfully driven to M1 and M2 phenotypes by delivery of either IFN-γ or IL-4, respectively. SCs incubated with M1 or M2 macrophages-derived MVs, the cell migration and proliferation were assessed, and expression levels of nerve growth factor (NGF) and Laminin were measured. A rat model of sciatic nerve was established and the effects of macrophages-derived MVs on nerve regeneration were investigated.

RESULTS:

M2-derived MVs elevated migration, proliferation, NFG and Laminin protein levels of SCs compared with M1-or M0-derived MVs. The relative expression levels of miR-223 were also increased in M2 macrophages and M2-derived MVs. Transfected M2 macrophages with miR-223 inhibitor then co-incubated with SCs, an inhibition of cell migration and proliferation and a down-regulated levels of NFG and Laminin protein expression were observed. In vivo, M2-derived MVs significantly increased the infiltration and axon number of SCs.

CONCLUSION:

M2-derived MVs promoted proliferation and migration of SCs in vitro and in vivo, which provided a therapeutic strategy for nerve regeneration.

KEYWORDS:

Macrophage phenotype; Microvesicles; Nerve regeneration; Schwann cell

PMID:
26499078
DOI:
10.1016/j.bbrc.2015.10.097
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center