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Genome Biol. 2015 Oct 23;16:236. doi: 10.1186/s13059-015-0797-8.

Promoter-like epigenetic signatures in exons displaying cell type-specific splicing.

Author information

1
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader, 88, 08003, Barcelona, Catalonia, Spain.
2
Graduate program in Areas of Basic and Applied Biology, Abel Salazar Biomedical Sciences Institute, University of Porto, 4099-003, Porto, Portugal.
3
Universitat Pompeu Fabra, Dr. Aiguader, 88, 08003, Barcelona, Catalonia, Spain.
4
Department of Genetics, Stanford University, 300 Pasteur Dr., Stanford, CA, 94305-5120, USA.
5
Institució Catalana de Recerca i Estudis Avançats, Pg Lluis Companys 23, 08010, Barcelona, Catalonia, Spain.
6
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader, 88, 08003, Barcelona, Catalonia, Spain. roderic.guigo@crg.cat.
7
Universitat Pompeu Fabra, Dr. Aiguader, 88, 08003, Barcelona, Catalonia, Spain. roderic.guigo@crg.cat.

Abstract

BACKGROUND:

Pre-mRNA splicing occurs mainly co-transcriptionally, and both nucleosome density and histone modifications have been proposed to play a role in splice site recognition and regulation. However, the extent and mechanisms behind this interplay remain poorly understood.

RESULTS:

We use transcriptomic and epigenomic data generated by the ENCODE project to investigate the association between chromatin structure and alternative splicing. We find a strong and significant positive association between H3K9ac, H3K27ac, H3K4me3, epigenetic marks characteristic of active promoters, and exon inclusion in a small but well-defined class of exons, representing approximately 4 % of all regulated exons. These exons are systematically maintained at comparatively low levels of inclusion across cell types, but their inclusion is significantly enhanced in particular cell types when in physical proximity to active promoters.

CONCLUSION:

Histone modifications and other chromatin features that activate transcription can be co-opted to participate in the regulation of the splicing of exons that are in physical proximity to promoter regions.

PMID:
26498677
PMCID:
PMC4619081
DOI:
10.1186/s13059-015-0797-8
[Indexed for MEDLINE]
Free PMC Article

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