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Arch Virol. 2016 Jan;161(1):219-22. doi: 10.1007/s00705-015-2630-9. Epub 2015 Oct 23.

Complete genome sequence of a distinct calla lily chlorotic spot virus isolated in mainland China.

Author information

1
College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China.
2
Center of Crop Science Experimental Teaching, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
3
Yunnan Vocational and Technical College of Agriculture, Kunming, 650201, China.
4
College of Life Science and Technology, Honghe University, Mengzi, 661199, China.
5
College of Tropical Agriculture and Human Resources, University of Hawaii, Honolulu, HI, 96822, USA.
6
Center of Crop Science Experimental Teaching, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China. liuyating66@163.com.

Abstract

The first complete genome sequence of calla lily chlorotic spot virus (CCSV) from Lijiang in northwestern Yunnan Province was obtained using RT-PCR with designed primers. The genome of CCSV isolate LJ-1-Yunnan is tripartite. The small (S) RNA is 3182 nucleotides (nt) in length and encodes a nonstructural protein (NSs, 1383 nt) and a nuclear nucleocapsid (N, 834 nt), separated by an 836-nt intergenic region (IGR). The medium (M) RNA is 4749 nt in length and encodes a nonstructural movement protein (NSm, 930 nt) and a glycoprotein (GnGc, 3,372 nt), also separated by a 349-nt IGR. The large (L) RNA is 8912 nt in length and encodes a predicted RNA-dependent RNA polymerase (RdRp, 8652 nt). The nucleotide sequences of the three viral RNA segments are 92-94 % identical to the published CCSV genome sequence, and the amino acid sequences of the encoded proteins are 96-98 % identical. However, the IGRs of the S and M RNAs are less similar, with 86 and 72 % identity, respectively. Genome sequence comparisons and phylogenetic analysis indicate that the Lijiang CCSV isolate is a unique tospovirus isolate that differs from CCSV isolates in other geographic regions.

PMID:
26497175
DOI:
10.1007/s00705-015-2630-9
[Indexed for MEDLINE]

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