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J Infect Dis. 2016 Mar 1;213(5):856-65. doi: 10.1093/infdis/jiv506. Epub 2015 Oct 22.

HlyF Produced by Extraintestinal Pathogenic Escherichia coli Is a Virulence Factor That Regulates Outer Membrane Vesicle Biogenesis.

Author information

1
Division of Bioenvironmental Science, Frontier Science Research Center.
2
INSERM, UMR1043 INRA, USC 1360 CNRS, UMR5282 Université de Toulouse, UPS, Centre de Physiopathologie de Toulouse Purpan.
3
Institut National de la Recherche Scientifique, Institut Armand-Frappier, Laval, Canada.
4
INRA, UMR1282, Nouzilly, France.
5
Division of Bioenvironmental Science, Frontier Science Research Center Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Japan.
6
INSERM, UMR1043 INRA, USC 1360 CNRS, UMR5282 Université de Toulouse, UPS, Centre de Physiopathologie de Toulouse Purpan CHU Toulouse, Hôpital Purpan, Service de bactériologie-hygiène.

Abstract

Escherichia coli can cause extraintestinal infections in humans and animals. The hlyF gene is epidemiologically associated with virulent strains of avian pathogenic E. coli and human neonatal meningitis-associated E. coli. We demonstrated that culture supernatants of E. coli expressing HlyF induced autophagy in eukaryotic cells. This phenotype coincided with an enhanced production of outer membrane vesicles (OMVs) by bacteria expressing HlyF. The HlyF protein displays a predicted catalytic domain of the short-chain dehydrogenase/reductase superfamily. This conserved domain was involved the ability of HlyF to promote the production of OMVs. The increased production of OMVs was associated with the release of toxins. hlyF was shown to be expressed during extraintestinal infection and to play a role in the virulence of extraintestinal pathogenic E. coli in a chicken model of colibacillosis. This is the first evidence that pathogenic bacteria produce a virulence factor directly involved in the production of OMVs.

KEYWORDS:

HlyF; OMV; pathogenesis

PMID:
26494774
DOI:
10.1093/infdis/jiv506
[Indexed for MEDLINE]

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