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J Dent Res. 2016 Feb;95(2):206-14. doi: 10.1177/0022034515610748. Epub 2015 Oct 22.

Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration.

Author information

1
Department of Physiology, Keio University School of Medicine, Tokyo, Japan Department of Dentistry and Oral Surgery, Keio University School of Medicine, Tokyo, Japan Department of Dentistry and Oral Surgery, Kawasaki Municipal Kawasaki Hospital, Kanagawa, Japan.
2
Department of Physiology, Keio University School of Medicine, Tokyo, Japan Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
3
Department of Neurology, Keio University School of Medicine, Tokyo, Japan.
4
Department of Dentistry and Oral Surgery, Keio University School of Medicine, Tokyo, Japan Division of Molecular and Regenerative Prosthodontics, Graduate School of Dentistry, Tohoku University, Miyagi, Japan.
5
Centre for Liver Research, NIHR Biomedical Research Unit, University of Birmingham, Birmingham, UK.
6
Department of Dentistry and Oral Surgery, Keio University School of Medicine, Tokyo, Japan.
7
Department of Dentistry and Oral Surgery, Kawasaki Municipal Kawasaki Hospital, Kanagawa, Japan.
8
Department of Biochemistry and Biophysics, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
9
Department of Physiology, Keio University School of Medicine, Tokyo, Japan.
10
Department of Physiology, Keio University School of Medicine, Tokyo, Japan Department of Cancer Biology, Faculty of Medicine, Shimane University, Shimane, Japan matsuzak@med.shimane-u.ac.jp.

Abstract

Human dental pulp stem/progenitor cells (hDPSCs) are attractive candidates for regenerative therapy because they can be easily expanded to generate colony-forming unit-fibroblasts (CFU-Fs) on plastic and the large cell numbers required for transplantation. However, isolation based on adherence to plastic inevitably changes the surface marker expression and biological properties of the cells. Consequently, little is currently known about the original phenotypes of tissue precursor cells that give rise to plastic-adherent CFU-Fs. To better understand the in vivo functions and translational therapeutic potential of hDPSCs and other stem cells, selective cell markers must be identified in the progenitor cells. Here, we identified a dental pulp tissue-specific cell population based on the expression profiles of 2 cell-surface markers LNGFR (CD271) and THY-1 (CD90). Prospectively isolated, dental pulp-derived LNGFR(Low+)THY-1(High+) cells represent a highly enriched population of clonogenic cells--notably, the isolated cells exhibited long-term proliferation and multilineage differentiation potential in vitro. The cells also expressed known mesenchymal cell markers and promoted new bone formation to heal critical-size calvarial defects in vivo. These findings suggest that LNGFR(Low+)THY-1(High+) dental pulp-derived cells provide an excellent source of material for bone regenerative strategies.

KEYWORDS:

THY-1; bone regeneration; flow cytometry; isolation; low-affinity nerve growth factor receptor; transplantation

PMID:
26494655
DOI:
10.1177/0022034515610748
[Indexed for MEDLINE]

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