Format

Send to

Choose Destination
Fertil Steril. 2016 Jan;105(1):58-64. doi: 10.1016/j.fertnstert.2015.09.041. Epub 2015 Oct 19.

Relationship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional semen parameters, and recurrent pregnancy loss.

Author information

1
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia. Electronic address: zidiines@hotmail.com.
2
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.
3
Department of Obstetrics and Gynecology, Farhat Hached University Hospital, Sousse, Tunisia.

Abstract

OBJECTIVE:

To study the possible relationship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional semen parameters, and recurrent pregnancy loss (RPL).

DESIGN:

Descriptive study.

SETTING:

University-affiliated tertiary teaching.

PATIENT(S):

A total of 22 couples with history of RPL and 20 fertile men.

INTERVENTION(S):

Semen samples from case and control men were examined for differences in semen parameters, DNA fragmentation, chromatin condensation, and sperm aneuploidy.

MAIN OUTCOME MEASURE(S):

Sperm DNA and chromatin integrity and sperm aneuploidy.

RESULT(S):

Sperm progressive motility (30.2% vs. 51.5%) was significantly lower and abnormal morphology (74.8% vs. 54.2%) was significantly higher in the RPL group versus the control group, respectively. The percentage of fragmented DNA was significantly increased in the RPL group (17.1% vs. 10.2%) as well as the rate of spermatozoa with nuclear chromatin decondensation (23.6% vs. 11.8%). There was a significantly higher sperm aneuploidy rate among the RPL group as well.

CONCLUSION(S):

The increase in abnormal sperm parameters, sperm DNA fragmentation, nuclear chromatin decondensation, and sperm aneuploidy suggest possible causes of unexplained RPL.

KEYWORDS:

DNA damage; Spermatozoa; aneuploidy; pregnancy loss

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center