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Environ Toxicol Pharmacol. 2015 Nov;40(3):870-5. doi: 10.1016/j.etap.2015.09.012. Epub 2015 Sep 28.

Chemopreventive activity of grape juice concentrate (G8000TM) on rat colon carcinogenesis induced by azoxymethane.

Author information

1
Oncology Division, Medicine Department, Universidade Federal de São Paulo UNIFESP/EPM, SP, Brazil.
2
Department of Pathology, Universidade Federal de São Paulo UNIFESP/EPM, SP, Brazil.
3
Department of Biosciences, Universidade Federal de São Paulo UNIFESP, Santos, SP, Brazil. Electronic address: daribeiro@unifesp.br.

Abstract

Colorectal cancer is the third most common cancer worldwide in both sexes, with similar geographic patterns between genders. This neoplasm has good prognosis if the disease is diagnosed at early stages. The aim of this study was to evaluate the effect of red grape juice on the expression of COX-2 and Ki-67 expression following colon carcinogenesis induced by azoxymethane (AOM). Thirty-five rats were randomly distributed into seven groups (n=5 per group): G1: SHAM or negative control received only saline; G2 (positive control): animals received 15 mg/kg AOM; G3: animals received 1% red grape juice 2 weeks before the administration of AOM; G4: animals received 2% red grape juice 2 weeks before the administration of AOM; G5: animals received 1% red grape juice 4 weeks after the last administration of AOM; G6: animals received 2% red grape juice 4 weeks after the last administration of AOM; G7: animals received only 2% red grape juice. COX-2 mRNA expression was reduced in animals treated with 1% red grape juice before AOM induction or 2% red grape juice after AOM induction. COX-2 immunoexpression was also reduced to groups treated with red grape juice at 1% before and after AOM induction or 2% red grape juice after AOM induction. Decreased immunoexpression of Ki-67 positive cells was observed in animals treated with 1% grape juice before AOM-treated animals. Taken together, grape juice concentrate is able to exert some chemopreventive activity on rat colon carcinogenesis.

KEYWORDS:

Cell proliferation; Colon carcinogenesis; Cyclooxygenase 2; Grape juice; Rat

PMID:
26492449
DOI:
10.1016/j.etap.2015.09.012
[Indexed for MEDLINE]

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