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Eur Heart J. 2017 Mar 1;38(9):675-686. doi: 10.1093/eurheartj/ehv520.

Notch-Tnf signalling is required for development and homeostasis of arterial valves.

Author information

1
Department of Genetics, Pediatrics, and Medicine (Cardiology), Wilf Cardiovascular Research Institute, Albert Einstein College of Medicine, Price 420, 1301 Morris Park Avenue, Bronx, NY 14061, USA.
2
Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
3
Division of Molecular Cardiovascular Biology, Cincinnati Children's Medical Centre, Cincinnati, OH, USA.
4
Institute for Aging Research, Albert Einstein College of Medicine, Bronx, NY, USA.
5
Department of Medicine and Geriatrics (Cardiology), First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
6
Department of Internal Medicine, Saint Louis University, St. Louis, MO, USA.
7
Department of Biomedicine, University of Basel, Basel, Switzerland.
8
Department of Paediatrics, Indiana University, Indianapolis, IN, USA.

Abstract

Aims:

Congenital anomalies of arterial valves are common birth defects, leading to valvar stenosis. With no pharmaceutical treatment that can prevent the disease progression, prosthetic replacement is the only choice of treatment, incurring considerable morbidity and mortality. Animal models presenting localized anomalies and stenosis of congenital arterial valves similar to that of humans are critically needed research tools to uncover developmental molecular mechanisms underlying this devastating human condition.

Methods and results:

We generated and characterized mouse models with conditionally altered Notch signalling in endothelial or interstitial cells of developing valves. Mice with inactivation of Notch1 signalling in valvar endothelial cells (VEC) developed congenital anomalies of arterial valves including bicuspid aortic valves and valvar stenosis. Notch1 signalling in VEC was required for repressing proliferation and activating apoptosis of valvar interstitial cells (VIC) after endocardial-to-mesenchymal transformation (EMT). We showed that Notch signalling regulated Tnfα expression in vivo, and Tnf signalling was necessary for apoptosis of VIC and post-EMT development of arterial valves. Furthermore, activation or inhibition of Notch signalling in cultured pig aortic VEC-promoted or suppressed apoptosis of VIC, respectively.

Conclusion:

We have now met the need of critical animal models and shown that Notch-Tnf signalling balances proliferation and apoptosis for post-EMT development of arterial valves. Our results suggest that mutations in its components may lead to congenital anomaly of aortic valves and valvar stenosis in humans.

KEYWORDS:

Congenital anomalies of arterial valves; Notch; Tnf; Valvar aortic stenosis

PMID:
26491108
PMCID:
PMC5837252
DOI:
10.1093/eurheartj/ehv520
[Indexed for MEDLINE]
Free PMC Article

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