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Bioinformatics. 2016 Feb 1;32(3):459-61. doi: 10.1093/bioinformatics/btv571. Epub 2015 Oct 19.

RNA structure framework: automated transcriptome-wide reconstruction of RNA secondary structures from high-throughput structure probing data.

Author information

1
Human Genetics Foundation (HuGeF), 10126 Torino, Italy and Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, 10123 Torino, Italy.
2
Human Genetics Foundation (HuGeF), 10126 Torino, Italy and.

Abstract

SUMMARY:

The rapidly increasing number of discovered non-coding RNAs makes the understanding of their structure a key feature toward a deeper comprehension of gene expression regulation. Various enzymatic- and chemically- based approaches have been recently developed to allow whole-genome studies of RNA secondary structures. Several methods have been recently presented that allow high-throughput RNA structure probing (CIRS-seq, Structure-seq, SHAPE-seq, PARS, etc.) and unbiased structural inference of residues within RNAs in their native conformation. We here present an analysis toolkit, named RNA Structure Framework (RSF), which allows fast and fully-automated analysis of high-throughput structure probing data, from data pre-processing to whole-transcriptome RNA structure inference.

AVAILABILITY AND IMPLEMENTATION:

RSF is written in Perl and is freely available under the GPLv3 license from http://rsf.hugef-research.org.

CONTACT:

salvatore.oliviero@hugef-torino.org

SUPPLEMENTARY INFORMATION:

Supplementary data are available at Bioinformatics online.

PMID:
26487736
DOI:
10.1093/bioinformatics/btv571
[Indexed for MEDLINE]
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